English
聯系我們
網站地圖
郵箱
舊版回顧



城市環境所利用單細胞拉曼光譜研究土壤解磷微生物取得進展

文章來源:城市環境研究所   發布時間:2019-05-10  【字號:     】  

  磷是一種至關重要但不可再生的資源。磷肥是作物生長必須三大營養元素(氮、磷、鉀)之一,但磷礦是有限資源,據估計,以目前的磷礦儲備,大概還能繼續開采100年,因此,磷又被稱為“正在消失的元素”。隨著長期大量的磷肥施用,土壤中積累了大量固定態磷,大大降低了磷素的生物有效性,加劇了磷資源短缺的問題,對土壤固定態磷的活化成為未來發展可持續農業和磷肥利用的重要戰略。

  微生物是土壤元素循環的發動機,解磷微生物可以通過釋放有機酸溶解土壤中的鈣磷,或釋放活性磷酸酶活化土壤中的有機磷。利用解磷微生物釋放土壤中被固定的磷,提升磷的生物有效性,對磷素的可持續利用和緩解磷礦枯竭危機具有至關重要的作用。然而,環境微生物的大量不可純培養性,對開發利用微生物資源帶來了很大的挑戰。從單細胞水平上研究環境微生物可克服純培養或富集培養的限制,實現在環境介質下的原位研究,拉曼光譜可在單細胞水平上對微生物進行無損檢測。

  中國科學院城市環境研究所朱永官研究團隊發展了利用單細胞拉曼結合重水標記篩選高活性土壤解磷微生物的新方法。該方法可以無損檢測土壤環境中不可培養的微生物,從而挖掘出具有強解磷功能的微生物。方法原理是:在僅固定態無機磷(如難溶磷酸三鈣)或有機磷(如卵磷脂)存在的環境中,具有解磷功能的微生物由于仍具活性,甚至生長繁殖能力,因此能同化重水中的氘,產生特征的C-D拉曼峰。解磷微生物與非解磷微生物在C-D拉曼峰存在巨大的強度差異,解磷能力越強的微生物具有越強的C-D拉曼峰,利用這一特性可以靈敏快速地篩選出土壤中的解磷微生物。重水標記由于不改變環境營養底物庫,可更真實反映土壤環境中的解磷微生物。上述工作促進了對未知環境菌群的深入認識,尤其是磷循環功能菌及其活性的深入解析。研究成果以D2O Isotope Labeling Approach to Probing Phosphate Solubilizing Bacteria in Complex Soil Communities by Single-Cell Raman Spectroscopy 為題發表在國際期Analytical Chemistry,城市環境所博士生李弘哲為第一作者,研究員崔麗為通訊作者。該研究得到科技部國家重點研發計劃和國家自然科學基金等的資助。

  文章鏈接 

 

土壤解磷菌拉曼鑒定流程

 

無磷土壤中培養24小時的土壤細菌的單細胞拉曼光譜




(責任編輯:葉瑞優)

附件:

專題推薦

相關新聞


© 1996 - 中國科學院 版權所有 京ICP備05002857號  京公網安備110402500047號  聯系我們

地址:北京市三里河路52號 郵編:100864

华为彩票www.hw8222.com www.130376.com | www.655557.com | 890876.cc | www.7dl.com | www.46126.com | www.151696.com | www.858459.com | www.76138.cc | www.506540.com | www.080841.com | www.356747.com | www.537110.com | www.873546.com | www.353653.com | www.682109.com | www.720079.com | www.868618.com | www.20je.com | www.177844.com | www.377576.com | www.655522.com | www.901779.com | www.976719.com | www.871664.com | www.722661.com | www.861972.com | 010768.com | www.4690.top | www.17622.cc | www.214323.com | www.632315.com | www.981846.com | 292002.com | www.557392.com | www.788391.com | 949503.com | www.1654.org | www.55676.com | www.s90.cc | www.128390.com | www.624163.com | www.912390.com | www.02434.com | www.457728.com | www.502399.com | www.749979.com | www.872346.com | www.33fk.com | www.8639.xyz | www.9193.cm | www.18866.cc | www.999563.com | www.111714.com | www.853938.com | 5115.hk | www.2gi.com | www.025787.com | www.dv8828.com | www.9916.cn | www.54547.cc | www.444622.com | www.791605.com | www.00ir.com | www.555781.com | www.754870.com | www.752719.com | www.642999.com | www.835929.com | www.43xg.com | www.66760.com | www.467557.com | www.649955.com | 755004.com | www.066114.com | www.580609.com | www.761491.com | www.201233.com | kq7.com | www.vn01.com | www.784.tv | www.59323.cc | 350101.com | www.82xk.com | www.552348.com | www.667293.com | www.g28.cc | www.37171.com | www.165981.com | www.480863.com | www.527958.com | www.664545.com | 185331.com | www.01783.com | 668460.com | 194303.com | www.33bp.com | www.39bp.com | www.6377.net | www.595127.com | www.767011.com | www.zc18.com | www.710658.com | www.987444.com | 751642.com | www.315.live | 625130.com | www.650961.com | 707951.com | 676804.com | www.51bw.com | www.130592.com | www.739440.com | www.ng82.com | 彩世界www.99788h.com | 961439.com | www.980453.com | www.040535.com | www.4ev.cc | www.1976.org | www.151920.com | www.287011.com | www.852939.com | www.691157.com | 963704.com | 豪彩VIPwww.9fk.com | www.a72.top | www.32oo.com | www.a74.top | www.791108.com | www.577008.com | www.0669.cc | www.103503.com | www.089912.com | 亿彩堂www.87668j.com | www.90ct.com | www.18004.com | www.177236.com | www.433311.com | 639203.com | 大玩家彩票www.929115.com | www.29773.com | 9865.biz | www.994670.com | www.lu45.com | www.93yh.com | www.68zr.com | www.34qd.com | www.19rl.com | www.05ju.com | www.3156.vip | www.9747.vip | www.285367.com | www.824900.com | www.5022.biz | 338495.com | www.vr53.com | www.22ur.com | www.90ix.com | www.5475.me | 8号彩票www.60108k.com | 486368.com | www.825493.com | www.8fm.com | www.339.la | www.42768.com | www.91568.com | www.816295.com | www.006356.com | www.438384.com | www.732302.com | www.953798.com | www.636188.com | www.766080.com | www.936151.com | www.973426.com | www.854279.com | www.rm06.com | www.ud58.com | www.2638.org | www.3380.wang | 586305.com | www.9088.biz | 01lh.com | 5327.biz | www.06zp.com | www.877486.com | 534422.com | www.50fd.com | www.34221.com | www.522288.com | www.999169.com | www.mr49.com | www.4132.biz | www.0820.org | www.036522.com | www.01251.com | www.200659.com | www.cp3775.com | www.6639.top | www.61108.com | www.422118.com | www.950336.com | www.990999.com | 026728.com | www.0182.top | www.737967.com | www.738628.com | www.22il.com | www.4967.top | www.9682.wang | www.005043.com | www.229010.com | www.339161.com | www.kx15.com | www.952141.com | www.125041.com | www.28151.cc | www.55282.com | www.327269.com | www.670737.com | www.503608.com | www.659123.cc | 370062.com | www.73684.com | www.334857.com | www.101851.com | www.098608.com | www.356204.com | www.178210.com | www.126476.com | www.131006.cc | www.568037.com | www.579382.com | www.456746.com | www.926516.com | www.776329.com | www.679735.com | www.659183.com | www.952059.com | www.821043.com | www.727535.com | www.650692.com | www.674701.com | www.931133.com | 402097.com | 奔驰彩票www.29178f.com | 55834.com | 370384.com | www.1hi.cc | www.75uq.com | www.588509.com | 幸运彩票www.774808.com | www.od02.com | 201942.com | 411899.com | 多宝彩票www.75538b.com | www.74nt.com | www.75319.com | www.326363.com | www.536470.com | www.731430.com | 974576.com | 598552.com | www.6000.tv | www.016193.com | www.049797.com | www.091188.com | www.441014.com | www.724408.com | www.5260.vip | www.021673.com | www.010370.com | www.129722.com | www.229932.com | www.259698.com | www.510726.com | 020455.com--020455.com | www.qv02.com | www.bm42.com | 676281.com | 3683.biz | 234224.com | 964225.com | 230699.com | pk79.com--pk79.com | 540208.com | 961942.com | 体彩网www.3890n.com | www.cai077.com | 如意彩票www.833585.com | www.05vp.com | www.0zi.com | www.2213.vip | www.997495.com | 948950.com | www.9jr.com | www.638.hk | www.48140.com | www.232785.com | www.293111.cc | www.230183.com | www.266386.com | 691710.com | 655849.com | www.812858.com | www.779409.com | www.758433.com | www.671119.com | www.cp61.com | www.c33.vip | www.8128.vip | www.31655.cc | www.28116.cc | www.44494.cc | www.678941.com | www.cp470.com | www.0118.org | www.096960.com | www.369961.com | www.356694.com | www.554425.com | www.0468.xyz | 779267.com | www.1838.space | 288685.com | www.201530.com | www.262256.com | www.295379.com | 388706.com | 131805.com | www.958171.com | 550569.com | 946858.com | www.123227.com | www.401296.cc | 63sb.com | www.wh95.com | www.56oq.com | www.960378.com | www.883757.com | 254752.com | 930316.com | www.518814.com | www.52bg.com | www.3793.pw | 305048.com | C9999www.318548.com | www.pm48.com | www.7855.loan | www.19py.com | www.294442.com | www.787538.com | 025834.com | www.981228.com | www.926287.com | www.842305.com | 830.cc | 487021.com | www.973504.com | 华彩网www.www.hcw332.com | www.5508.cn | www.117643.com | www.62556.com | www.477333.com | 981431.com | www.756024.com | 047808.com | www.28ve.com | www.25144.com | www.787018.com | www.794800.com | www.982828.com | www.590992.com | www.483465.com | www.947832.com | www.954010.com | www.939506.com | www.215611.com | www.286357.com | www.110271.com | www.459638.com | www.519716.com | www.802454.com | www.989838.com | 999126.cc | 133272.com | 949107.com | www.231494.com | www.637062.com | cx28.cc | 29kj.com | www.599032.com | www.588642.com | www.522446.com | www.618986.com | www.942314.com | 413994.com | www.653393.com | www.24138.com | www.16573.cc | www.11906.com | www.92957.cc | www.012132.com | www.248359.com | www.982.cm | www.55ud.com | www.iu54.com | www.df02.com | www.237848.com | www.552082.com | www.215115.com | www.931200.com | www.16128.cc | www.51259.cc | www.cg6.com | www.575617.com | www.843639.com | www.b95.xyz | www.371265.com | www.796122.com | www.977691.com | www.747516.com | www.64036.com | www.2186.net | www.0499.xyz | www.005082.com | www.73327.cc | www.358727.com | 964350.com | 043838.com | yj3.cc | 033059.com | 608221.com | 057209.com | 870188.com | www.178441.com | www.364618.com | www.258738.com | www.009665.com | www.51197.cc | www.051222.com | 236549.com | www.42eu.com | www.94df.com | www.96510.com | www.303552.com | www.652779.com | www.906638.com | 大赢家彩票www.599643.com | www.599099.com | 099059.com | 384977.com | 中彩网www.61233s.com | www.4593.vip | 53373.com | www.ys90.com | www.38116.cc | www.172620.com | www.15671.com | www.8686.pw | www.6066.top | www.8682.cc | www.28528.cc | www.68137.cc | www.363182.com | 121057.com | www.7567.top | www.3691.biz | www.1900.space | www.97497.com | www.549794.com | 857292.com | www.47052.com | www.61257.cc | www.423122.com | www.513998.com | www.667856.com | www.587179.com | 994073.com | 328206.com | 796425.com | 203961.com | www.pr90.com | www.9308.org | www.179102.com | www.274685.com | www.8760.in | www.202252.com | www.97419.com | www.230402.com | www.cai3011.com | www.1915.cm | www.06122.com | www.287297.com | www.je56.com | www.488066.cc | www.283208.com | 026031.com | www.xh17.com | www.hq45.com | www.083047.com | www.291952.com | www.303084.com | www.313603.com | www.270319.com | www.529390.com | www.408844.com | www.767572.com | www.659037.com | www.905292.com | www.wc99.cc | 百姓彩票www.bxcp3.com | 254273.com | www.108551.com | www.781611.com | 770373.com | 935770.com | 532440.com | www.544552.com | www.388684.com | www.678296.com | www.524138.cc | www.582207.com | www.865350.com | 4612.biz | 500彩票www.397477.com | 福彩www.26878w.com | 665423.com | www.lq00.com | www.723787.com | 290157.com | 357148.com | www.485036.com | www.531822.com | www.699290.com | 149975.com | www.yd.com | 天天中彩票www.969751.com | www.9296.online | www.808290.com | www.8269.loan | www.96269.cc | www.301610.com | www.791331.com | 885487.com | www.9127.biz | www.0191.top | www.484422.com | www.281131.com | www.017.loan | www.44246.com | www.670581.com | www.947879.com | www.212662.com | www.893692.com | www.009900.com | www.746722.com | 870443.com | 406951.com | www.8817.net | www.6384.vip | www.098955.com | www.060652.com | www.96189.cc | www.32939.cc | www.521334.com | www.623026.com | www.vi24.com | www.gd50.com | www.206860.com | www.012536.com | www.108738.com | 531.site | wn54.com | www.020.tv | www.298435.com | www.550560.com | www.ih05.com | 324428.com | www.731374.com | www.716363.com | www.191837.com | www.64qb.com | www.935107.com | 773359.com | www.091034.com | 5098.biz | www.525868.com | www.183216.com | www.655299.com | www.cai6322.com | www.kn38.com | www.5415.pro | www.25220.com | www.33348.cc | www.90jr.com | www.9988.in | www.li75.com | www.2893.cn | www.63so.com | www.659643.com | 爱彩www.6888ac.com | www.254870.com | www.171977.com | www.928839.com | www.817383.com | www.655937.com | www.808860.com | www.qs87.com | www.kx53.com | www.ba74.com | 853649.com | www.886826.com | www.5575.xyz | www.64nh.com | www.1512.cn | www.889013.com | 004817.com | 072320.com | www.3ai.cc | www.40598.com | www.139907.com | www.609411.com | www.63oo.com | www.85772.com | www.23767.com | www.921716.com | www.206997.com | 89790.com | www.582007.com | www.du60.com | www.751915.com | www.026483.com | www.37357.com | www.9707.cm | www.126843.com | 凤凰彩票www.66652g.com | 433652.com | www.892773.com | www.960949.com | 954113.com | www.10cp.cc | www.il83.com | www.009631.com | www.000140.com | www.017880.com | www.04593.com | www.cp5993.com | 大玩家彩票www.84499i.com | www.490958.com | 500彩票www.52072r.com | 万利www.32123r.com | www.rc70.com | www.hy15.com | www.8235.cm | www.200331.com | www.340229.com | www.361779.com | www.058082.com | www.qg90.com | 大赢家彩票网www.544090.com | 079172.com | 729871.com | 324687.com | www.876757.com | www.287234.com | www.801000.com | www.948707.com | www.750345.com | www.775330.com | 984372.com | www.923433.com | www.059829.com | www.079994.com | www.553548.com | www.820667.com | www.617113.com | www.552730.com | www.747005.com | www.p13.me | www.86167.com | www.6865.vip | www.80uk.com | 大赢家彩票平台www.109027.com | 641904.com | www.977806.com | 611867.com | th04.com | 223048.com | 549139.com | www.866550.com | www.971.tv | www.042977.com | 365520.cc | www.fw8.com | www.403.tv | www.9956.top | www.376009.com | www.hx16.com | www.779839.com | 499541.com | www.zz14.cc | www.2164.vip | www.13ix.com | www.490769.com | www.818714.com | www.947932.com | www.218020.com | www.617747.com | www.11350.com | www.005.net | www.102272.com | www.678516.com | 65553.cc | www.270093.com | www.176331.com | www.871744.com | 814175.com | 301635.com | www.801751.com | www.851877.com | 671402.com | www.132901.com | www.303511.com | 386477.com | www.da33.com | 370325.com | www.8190.in | www.88zx.com | www.253944.com | www.324408.com | www.525980.com | 495338.com | 355849.com | www.982209.com | www.816729.com | www.005470.com | www.902.org | www.024696.com | www.93218.com | www.2ni.com | 天下彩www.890994.com | 021496.com | www.232040.com | www.2782.net | www.883708.com | www.766063.com | www.638236.com | www.421921.com | www.355787.com | www.756689.com | 532181.com | 771440.com | www.937949.com | www.375892.com | www.696422.com | www.426622.com | 856341.com | 772948.com | www.005900.com | www.00309.com | 775457.com | 14552.com | 504896.com | wl36.com | www.84cq.com | www.581128.com | www.526941.com | www.587681.com | www.5255.biz | www.14oq.com | www.57sb.com | www.ec72.com | www.08iz.com | www.013700.com | www.141598.com | www.263610.com | www.052891.com | www.087012.com | www.6237.net | www.s31.org | www.783381.com | www.654157.com | www.b95.pw | www.1308.cc | www.2221.biz | 802244.com | www.ny05.com | www.126.la | www.0839.cc | www.1360.vip | www.vn01.com | www.di48.com | www.ad98.com | 481361.com | www.678371.com | www.429038.com | www.438705.com | www.269882.com | www.063377.com | www.41995.com | www.7352.live | www.0043.pw | www.38675.cc | www.m30.com | www.733.in | www.xt20.com | www.01824.com | www.438456.com | www.359377.com | www.764186.com | www.3332.in | www.409338.com | 845545.com | 781460.com | www.004203.com | www.21278.com | www.39sp.com | www.285500.com | www.094959.com | www.54659.com | www.31712.cc | www.687169.com | www.60602.com | www.759126.com | www.921850.com | www.692585.com | www.501148.com | www.934709.com | www.5688.cn | www.99725.cc | www.57005.cc | www.193023.com | www.166353.com | www.68257.com | www.762.red | www.188259.com | www.703861.com | 254937.com | 589477.com | www.812927.com | www.517235.com | www.301286.com | www.827301.com | www.036895.com | www.339364.com | 233054.com | www.39681.cc | www.745669.com | 812475.com | 102308.com | www.090837.com | www.502863.com | www.872085.com | 07aj.com | 993524.com | 553506.com | 410811.com | www.811937.com | www.4822.cn | www.0004.xyz | www.89214.com | www.96738.com | www.78737.cc | www.308411.com | wo47.com | www.966908.com | www.538577.com | www.8su.cc | 436355.com | www.fc72.com | www.590825.com | www.958804.com | 184300.com | www.lx45.com | www.535129.com | www.51261.cc | www.52977.cc | www.6787.vip | www.613587.com | www.603537.com | www.889646.com | 863016.com | 947412.com | 52474.cc | 459653.com | www.87tw.com | 天下彩www.499680.com | www.546181.com | www.321134.com | www.829127.com | www.996034.com | 496518.com | www.2sg.cc | www.54sw.com | www.78387.cc | www.067790.com | www.810922.com | www.575672.com | n00852.com | www.003697.com | www.909540.com | www.560740.com | 804695.com | www.319006.com | www.46hn.com | www.005494.com | www.337333.com | www.29466.com | 91109.cc | 10340.com | www.656542.com | www.391844.com | 汇丰彩票www.977hf.com | 131204.com | www.vg59.com | 523502.com | www.ht30.com | www.5910.vip | www.13wk.com | www.5756.cm | www.061.biz | www.jo70.com | www.664096.com | 807936.com | www.947299.com | www.397824.com | www.959689.com | www.513655.com | www.267.cc | www.046845.com | www.664801.com | www.8238.wang | www.25937.com | www.6646.live | www.303688.com | 33118.com | www.nu22.com | 025780.com | www.44965.com | www.15247.com | www.89qh.com | www.p13.cc | www.028297.com | www.2111.me | www.95643.com | www.28482.com | 购彩网www.79gcw.com | www.13uj.com | www.eo49.com | www.001365.com | www.3650.cm | www.hv12.com | www.052575.com | www.702182.com | www.861059.com | www.cs39.com | 全民快乐8www.82676.com | www.zp87.com | www.224118.com | www.939725.com | www.tm83.com | www.2966.org | www.lk03.com | www.566267.com | www.13xm.com | www.747991.com | www.7092.biz | www.928557.com | www.003184.com | 588781.com | www.561507.com | www.028230.com | www.842.cm | www.8498.cc | www.540108.com | www.ss40.com | www.94ad.com | 003241.com | www.961937.com | www.wi46.com | www.761705.com | www.209913.com | www.584399.com | www.025535.com | www.899018.com | www.f56.xyz | www.pl17.com | 714832.com | 67566.com | www.6480.tv | www.50co.com | 91彩票网www.912254.com | www.gc84.com | 965416.com | 00589.com | www.459928.com | www.284938.com | www.06619.com | www.237500.com | www.050685.com | www.8557.in | www.848583.com | 744757.com--94877.com | www.257539.com | www.sha142.com | 273882.com | www.835923.com | www.8bx.com | 5034.biz | www.z41.me | www.654910.com | 500彩票网www.61655y.com | 500彩票www.701564.com | 880555.com | www.561277.com | www.070051.com | www.58971.com | www.gh09.com | 301126.com | www.963425.com | 博友彩www.977657.com | 439217.com | www.688486.com | www.676775.com | www.909038.com | www.va89.com | www.603655.com | www.vd07.com | www.637964.com | www.295999.com | www.53535.com | 965245.com | 501624.com | www.994230.com | www.666861.com | www.914511.com | www.179515.com | www.360118.com | www.660268.com | www.773835.com | 01399.com | 506952.com | www.639403.com | www.803881.com | www.545636.com | www.224508.com | www.1038.in | www.479116.com | 4899.biz | www.762300.com | www.620487.com | www.215111.cc | 733767.com | www.06222.com | www.13185.com | www.30383.com | www.8rz.com | www.786515.com | www.376757.com | www.318782.com | www.465143.com | www.330128.com | 大赢家彩票平台www.726703.com | 309762.com | 390609.com | www.883194.com | www.47wa.com | www.3690.vip | www.vl2.com | www.66yy.com | www.456379.com | 123412.cc | www.2328.biz | www.9710.org | www.6453.net | 博友彩www.866546.com | 034007.com | 083005.com | www.938522.com | www.qp29.com | 982376.com | www.642.mobi | www.979646.com | 497313.com | www.9210.vip | www.291372.com | www.012370.com | www.283628.com | www.819654.com | www.526065.com | www.306644.com | www.901904.com | www.775691.com | www.624918.com | www.374868.com | www.977365.cc | www.675785.com | www.533231.com | www.335168.com | www.130781.com | www.506289.com | www.142409.com | www.577755.com | www.149603.com | www.96752.cc | www.289766.com | www.880288.com | www.587206.com | www.289370.com | www.353034.com | 622503.com | 513455.com | www.109870.com | www.84xx.com | www.079049.com | www.866467.com | www.722199.com | www.567902.com | www.347822.com | www.7649.vip | www.666716.com | www.7235.cm | www.629418.com | www.120592.com | www.559001.com | www.915315.com | www.669335.com | www.222270.com | 397342.com | www.247607.com | www.108665.com | www.438484.com | www.700780.com | 872943.com | www.1512.pw | www.190950.com | www.954786.com | www.461673.com | www.5677.la | www.bi32.com | www.3856.biz | www.zm40.com | 874156.com | 560253.com | www.730978.com | www.616005.com | www.cp7587.com | www.7574.pw | www.5333.mobi | www.093785.com | www.124173.com | www.51621.com | www.971929.com | www.699572.com | www.7092.pw | www.16jw.com | 91584.cc | 258905.com | www.38sn.com | www.96861.cc | www.432727.com | 819761.com | www.469.cm | www.mp27.com | www.93542.com | www.710035.com | www.969000.com | www.150165.com | www.1039.vip | www.8115.cm | www.tq53.com | 694909.com | www.84nd.com | 865446.com | www.402.in | 274552.com | www.994881.com | www.609666.cc | www.9789.win | www.0285.cm | www.130779.com | www.238518.com | www.38094.com | www.277361.com | www.12283.com | www.oc77.com | 593802.com | www.oo31.com | 080691.com--163a.com | www.863981.com | www.755011.com | www.420061.com | www.989649.com | www.586952.com | www.542885.com | www.122429.com | www.tp29.com | 125109.com | www.67es.com | www.947764.com | www.873551.com | 大赢家彩票平台www.703193.com | rl35.com | 261079.com | www.930312.com | 923458.com | 皮皮彩www.153680.com | 752692.com | www.971166.com | www.588403.com | www.271037.com | www.2151.xyz | www.036317.com | 393371.com | 037771.com | 500彩票www.50064b.com | www.867387.com | www.629031.com | www.585695.com | www.987387.com | www.931335.com | www.739046.com | www.112841.com | www.905500.com | 242676.com | 718392.com | 118190.com | www.898327.com | www.910200.com | www.409995.com | www.570769.com | 杏彩www.00840h.com | www.629035.com | www.378179.com | www.362869.com | www.578827.com | www.52253.cc | www.065235.com | www.51186.com | www.109638.com | www.8700.loan | www.69pv.com | www.04148.cc | www.21bl.com | www.st79.com | www.62225.cc | 266806.com | www.850325.com | 730549.com | www.910706.com | www.386717.com | 104198.com | www.945106.com | 420069.com | 744064.com | www.963485.com | www.36ue.com | www.x87.top | www.130.tv | www.yg71.com | www.9082.biz | www.209321.com | www.397797.com | www.526225.com | www.875313.com | www.8219.cn | www.562727.com | www.003145.com | 137944.com | www.771571.com | www.692017.com | 553966.com | 20567.com | www.037024.com | 096894.com | www.870087.com | www.579107.com | www.772429.com | www.885265.com | www.021949.com | www.6oc.com | PK彩票www.623190.com | www.984838.com | www.788232.com | www.861370.com | www.6681.cn | 760497.com | www.751739.com | www.212684.com | www.068582.com | www.1979.top | www.cb32.com | www.qa73.com | 9a0w7.emu999.net | www.321449.com | www.857943.com | 天天彩www.87467.com | www.32624.com | www.132000.cc | www.097097.cc | www.595211.com | www.456265.com | www.252161.com | www.90608.com | www.ts18.com | www.97563.com | www.232323.com | www.93218.cc | www.761.me | www.16766.com | www.66722.com | www.19579.com | www.92us.com | www.963737.com | www.606968.com | www.028891.com | www.173700.com | 948013.com | www.882884.com | www.508888.com | www.399940.com | www.525785.com | 939945.com | www.28yn.com | www.870252.com | mn65.com | 670085.com | www.813396.com | www.887551.com | 968168.cc | www.991780.com | www.662101.com | www.801021.com | www.713882.com | www.380578.com | www.921371.com | www.504025.com | www.111337.com | www.535382.com | www.462422.com | www.593198.com | www.187601.com | www.630290.com | 551334.com | www.761012.com | 110416.com | www.711780.com | www.606750.com | www.073213.com | www.3694.biz | www.72097.com | www.689050.com | www.366927.com | 238897.com | www.069023.com | www.011465.com | www.wx77.com | 555106.com | www.35688.com | www.132813.com | www.791796.com | www.gl75.com | www.31vd.com | www.6083.vip | 951492.com | www.71290.com | www.188462.com | www.5375.cn | www.36mw.com | www.85um.com | 500彩票www.50024p.com | www.976009.com | www.47yb.com | www.31vs.com | www.578253.com | www.019475.com | www.004314.com | 715281.com | www.809034.com | www.626662.com | www.306929.com | www.320344.com | www.303918.com | www.694410.com | 中原彩票www.302591.com | www.973062.com | www.211695.com | www.96309.cc | www.929116.com | www.567182.com | www.610911.com | www.188809.cc | www.227911.com | www.88gb.com | www.9886.cm | www.588922.com | 954534.com | 420266.com | 920026.com | 713159.com | www.666742.com | 854810.com | www.25wh.com | www.96912.cc | www.443882.com | www.570355.com | www.91039.com | www.mp87.com | www.958974.com | www.84rj.com | 735927.com | www.ez49.com | www.782783.com | www.jv49.com | www.979176.com | www.654613.com | 088254.com | www.702896.com | www.767373.com | www.303928.com | www.9666.cc | www.9783.top | www.8657.cm | www.201715.cc | www.176979.com | 033965.com | www.931987.com | www.947417.com | 502076.com | www.644321.com | www.341623.com | www.07426.com | www.007388.com | www.wz52.com | 890775.com | 898766.com | www.932772.com | 579203.com | www.819278.com | 5913.biz | www.999186.com | www.320090.com | www.41694.com | www.22cd.com | www.qh57.com | 26681.com | cp5123.com | www.753302.com | www.698522.com | www.256167.com | www.405054.com | www.677977.com | 731354.com | www.pl66.com | www.2766.in | 218290.com | www.962269.com | www.vf08.com | www.gz94.com | 954696.com | 161772.com | www.577686.com | www.286999.cc | www.16jw.com | www.527560.com | 351610.com | 500彩票www.303471.com | www.774074.com | www.69dj.cc | www.991890.com | 308129.com | www.736762.com | 478978.com | www.686642.com | www.012825.com | www.981811.com | www.691173.com | www.209144.com | www.819013.com | www.241686.com | www.696708.com | www.225322.com | www.751027.com | www.od76.com | www.087920.com | www.092266.com | www.025579.com | www.3302.cc | www.zn67.com | 951716.com | www.453518.com | www.427168.com | www.127432.com | www.76ba.com | www.50045.cc | www.529956.com | www.111612.com | www.81ao.com | 351937.com | www.353785.com | 586306.com | www.689809.com | www.266642.com | www.8368.pw | 594187.com | www.41573.com | www.797.in | www.00ch.com | www.cai4200.com | www.978634.com | www.054891.com | www.427744.com | www.091132.com | www.228211.com | www.350532.com | www.219713.com | www.27937.cc | www.is04.com | 314050.com | www.587561.com | www.169092.com | 747457.com | www.371708.com | www.219357.com | www.4220.xyz | www.cp0378.com | www.832035.com | www.314499.com | www.78276.com | www.978910.com | www.820069.com | 066197.com | www.xb12.com | www.93bs.com | www.939945.com | www.275744.com | www.569083.com | www.cj9.com | www.609512.com | www.658501.com | 486308.com | 151303.com | www.661465.com | www.078035.com | www.wl71.cc | yz89.cc | www.539406.com | www.91193.cc | 755948.com | www.599848.com | 财神网www.29277q.com | 亿博彩票www.65yb.com | www.900052.com | www.220261.com | www.7942.cm | www.70815.com | www.071772.com | www.64ch.com | cp409.com | www.11481.com | www.633061.com | www.329653.com | 394823.com | 383138.com | www.801758.com | 538731.com | www.0371.vip | 381510.com | www.254454.com | jn61.com | www.764346.com | www.433655.com | www.85307.com | 978586.com | www.166.cn | www.35sv.com | www.349404.com | www.93122.cc | www.pz81.cc | www.84jv.com | 263333.com | www.835301.com | www.181871.com | www.669915.com | www.405178.com | www.579614.com | 301988.com | www.110841.com | www.13de.com | 993743.com | www.871170.com | www.429628.com | www.737562.com | www.180512.com | www.275358.com | 516004.com | www.854518.com | www.059484.com | www.23030.cc | www.010688.com | www.5983.biz | 华彩网www.hcw887.com | www.22ky.cc | www.60ky.com | 485901.com | www.961159.com | www.478894.com | www.80883.cc | www.4799.xyz | www.212229.com | www.638322.com | www.ov22.com | 开心彩票www.ck5589.com | www.356834.com | www.3827.cm | 981064.com | www.910768.com | www.775626.com | 222307.com | 437523.com | www.668080.com | www.cy63.com | www.557704.com | www.273228.com | 779958.com | www.776248.com | www.9163.cm | www.467357.com | www.35953.com | 6791.biz | www.7888.top | www.126064.com | www.9694.cm | www.nb87.com | 39333.com | www.26009.com | www.752487.com | www.072050.com | www.909772.com | www.008276.com | www.608153.com | www.076656.com | www.47cc.com | 852901.com | 36339.cc | 461188.com | www.36yw.com | www.257851.com | www.r43.cn |